Degree projects

We welcome students to perform master and bachelor projects in our group. One requirement to perform a project in our group is to have attended a course in cell culturing or the course BIOR21 Toxicology. If you are interested, please contact Stina Oredsson. Among available projects are the following.

DNA strand breaks induced by polyamine analogue treatment

Polyamine analogues are presently tested in clinical trials in the US. They show good anti-cancer efficiency in experimental cell and animal systems. In the clinic they have shown a very good toxicity profile, i.e. the toxic side effects are low. Thus, clinical studies are continuing. However, the exact mechanisms of action are not known and, to be able to develop these compounds, we urgently need to understand the molecular mechanisms. Cell cycle kinetic studies give at hand that one mechanism of action is the induction of DNA strand breaks. In this project, the student will treat different cell lines with polyamine analogues and investigate if DNA strand breaks are induced with the single cell gel electrophoresis assay.

Ubiquitin and induction of differentiation

Ubiquitin is most commonly known as an intracellular protein involved in protein degradation. We have identified ubiquitin in bovine milk what you purchase in the store. When we cultivated cells in the presence of ubiquitin, the rate of cell proliferation was reduced and this was particularly evident in a specific cancer form. In this very interesting project, the student will incubate the cells with ubiquitin and investigate differentiation. As the data are yet not published, the project will not yet be totally revealed on this home page. A putative student will get more information.

Chemotherapy and development of resistance

Chemotherapy often leads to development of resistance in treated cancer cells. In this project, we wish to investigate if cancer cells can develop resistance to polyamine analogues and if so under which conditions so that these conditions can be avoided in the clinic.

Multicellular spheroids of MCF-10A normal breast epithelial cells grown in normoxia, A, or hypoxia, B. Green fluorescence: CD44. Red fluorescence: CD24. Blue fluorescence: bisbenzimide-stained nuclei. The bar represents 10 micrometer.


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Last modified 13 Dec 2011

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