We use human cell lines of normal and cancer origin growing in culture as experimental system. We have vast experience with cell culturing and are giving courses in cell culturing at undergraduate and graduate levels. We have also given courses outside of the university context at various industries. If you are interested in a course in cell culturing on the theoretical and practical levels within or outside the university, please contact Stina Oredsson.
We use flow cytometry to study cell cycle phase distribution, cell cycle kinetics and the distribution of antigens in a cell populations.
The cell cycle phase distribution is determined by staining a cell population with the stochiometric DNA dye propidium idodide. The distribution of cells in G1/G0, S and G2/M phases is deduced from the DNA histogram obtained after flow cytzzzzzzometric analysis of the propidium idodide-stained cells.
The cell cycle phase distribution gives a static view of the population and may indicate kinetics. However, the true cell cycle kinetics cannot be deduced from a DNA histogram. Cell cycle kinetics is about rates, i.e. the lengths of the G1, S, G2 and M phases and the rates of transition between the G1 and S phases and S and G2 phases. Cell cycle kinetics is easily determined with a DNA bromodeoxyuridine flow cytometry method.
Flow cytometry is often used to identify different cell populations after labelling the cell surface with antibodies towards various cell surface proteins. We are using flow cytometry to identify cancer stem cells and also to investigate the process of mesenchymal to epithelial transition induced by treatment with chemotherapeutic drugs.
Treatment with chemotherapeutic drugs induces different kinds of damage in the cell. We are investigating single and double strand DNA damage using the Comet assay. We have also found that food components can stimulate DNA repair and this was evidenced using the Comet assay.
We routinely use Western blot to investigate the level of various proteins involved in cell cycle regulation and apoptosis.